The Sphericalplate 5D (SP5D) is a 3D cell culture plate for the formation of uniform, standardized, and size controlled spheroids in high quality and yield. It also allows you to effortlessly scale up and further translate into diagnostic or clinical applications. Simultaneously, it increases the reproducibility of subsequent tests, as you always start your experiments with identical initial conditions due to the low size variance of the cultured cell spheroids.

Per SP5D, you have twelve wells with 750 microwells each available. The patented geometry with special surface coating allows the integration of each individual cell into a 3D agglomerate in a controlled manner. Thus, up to 9,000 spheroids of uniform size grow while avoiding the formation of necrotic core. In addition, you have the option to conveniently perform monolayer cell culture for the comparison studies in the other twelve wells of the same plate.

Working with the ready-to-use SP5D is particularly user-friendly, so you will quickly get familiar with the handling of your new platform: The cultivation does not require any pre-treatment or centrifugation steps after seeding your cells. Changing the media is also particularly convenient by simple pipetting, as the height of the microwells has been designed to retain cell spheroids. In addition, the platform is compatible with standard automation units.

When developing the SP5D, the goal was to create the most realistic physiological environment possible for growing standardized spheroids with the possibility of cell-to-cell communication without external interfering signals. At the same time, the plate allows real-time imaging and monitoring within the platform, be scalable and convenient to use.

The global cell culture research market is changing from 2D to 3D. The Sphericalplate 5D is your solution covering the entire application range with unrivaled user advantages.

3D Cell Culture

Regenerative Medicine

Cancer Spheroid Research

3D Bio-Printing

Tissue Engineering

Drug Screening


Personalized Medicine

3D Stem Cell Culture

Cells Successfully Cultivated in SP5D

human embryonic stem cells human breast cancer cell line (MCF-7)
mouse embryonic stem cell line (HM-1) human A549 cell line from lung carcinoma
human bone marrow-derived mesenchymal stromal cells mouse 3T3 fibroblast cell line
human adipose-derived mesenchymal stromal cells human umbilical vein endothelial cell line (huVEC)
human islet cells human liver carcinoma cell line (HepG2)
rat islet cells human pluripotent stem cell line (Hs181)
mouse islet cells human osteosarcoma cell line (Saos-2)
human glioblastoma cell line human caucasian fetal lung cell line (WI 38)
human prostate cancer cell line human amniotic epithelial cells (hAEC)
human adrenal gland cancer cell line induced pluripotent stem cells (iPSCs)
human breast cancer line (BT20)

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